TITLE:
In Vitro Propagation Potential via Somatic Embryogenesis of the Two Maturing Early Cultivars of European Chestnut (Castanea sativa Mill.)
AUTHORS:
Mehmet Sezgin, Hatice Dumanoğlu
KEYWORDS:
Early Maturing Cultivars, in Vitro Propagation, Somatic Embryogenesis, Germinating
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.7,
May
10,
2016
ABSTRACT: Turkey
ranks the third in the production of chestnuts in the world having an important
place both in domestic and global markets. However, the chestnut production and
the number of trees have been diminishing in recent years. Therefore, in vitro propagation of the chestnut, in
addition to the classical propagation techniques, should be applied. Especially
the propogation of the early maturing cultivars and production of the quality
chestnuts will provide a better income to the producer. Here, somatic embryo
production and regeneration from the immature cotyledons of the early maturing
cultivars of the European chestnut (Castanea
sativa Mill), Haciibis and Karamehmet, were studied using the somatic
embryogenesis, one of the in vitro propagation techniques. To induce the somatic embryogenesis, 168 different
combinations were applied to both cultivars. The somatic embryogenesis rate in
Haciibis cultivar, in which the interactions were observed among the applications,
was found to be 9.9% while it was 11.1% for the Karamehmet cultivar.
Dessication, cold treatment, gibberellic acid (GA3) and
benzyladenine (BA) + naphthaleneacetic acid (NAA) applications were performed
on the regeneration of the somatic embryos, and 40% conversion to plant was
obtained with desiccation together with BA + NAA supplementation to the medium.