Article citationsMore>>

Speeckaert, M., Van Biesen, W., Delanghe, J., Slingerland, R., Wiecek, A., Heaf, J., Drechsler, C., Lacatus, R, Vanholder, R., Nistor, I. and for the European Renal Best Practice Guideline Development Group on Diabetes in Advanced CKD (2014) Are There Better Alternatives than Haemoglobin A1c to Estimate Glycaemic Control in the Chronic Kidney Disease Population? Nephrology Dialysis Transplantation, 1-11.

has been cited by the following article:

• TITLE: Effect of Hyperglycemia on Erythrocyte Carbonic Anhydrase and Lactic Acid in Type II Diabetic Subjects

  AUTHORS: Salihu Ismail Ibrahim, Ameh Danladi Amodu, Atawodi Sunday Ene-Ojo, Umar Alhaji Ismaila, Mohammed Fakhruddeen

  KEYWORDS: Carbonic Anhydrase, Lactic Acid, Glycated Hemoglobin, Type II Diabetes

  JOURNAL NAME: Journal of Diabetes Mellitus, Vol.6 No.2, May 6, 2016

  ABSTRACT: Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the reversible hydration of CO2 to and H+. CA plays a crucial role in CO2 transport, acid-base balance, and in linking local acidosis to O2 unloading from hemoglobin and also facilitates lactate shuttling across the monocarboxylate transporters (MCT). The study aimed to investigate the influence of hyperglycemia on erythrocyte carbonic anhydrase activity and lactic acid in type II diabetic patients. Method: Red blood cell carbonic anhydrase activity was determined in washed lysed-hemolysate by the action of the enzyme on the substrate p-nitrophenyl acetate. The absorbance of released p-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ion exchange method (Spectrum Diagnostic Kit). Blood glucose, lactate, cholesterol and triglyceride were determined using Accutrend GCT meter (Roche, Germany) with cobias®test strips. Results: The present study showed that hyperglycemia significantly (p