TITLE:
The Effects of a Hyperinsulinemic-Euglycemic Clamp on Milk Fat Synthesis and the Expression of Fat Synthesis-Related Genes in the Mammary Gland Tissues of Lactating Goats
AUTHORS:
Xueyan Lin, Guimei Liu, Yabin Zhang, Zhengui Yan, Qiuling Hou, Kerong Shi, Yun Wang, Zhonghua Wang
KEYWORDS:
Insulin, Milk Fat, Goat, Lactation
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.7 No.4,
April
29,
2016
ABSTRACT: To determine whether insulin exerts an effect on milk fat yield through the direct regulation of
milk fat synthesis in the mammary gland, the hyperinsulinemic-euglycemic clamp procedure was
performed in lactating goats in the present study. The effects of the hyperinsulinemic-euglycemic
clamp on milk yield, milk composition, milk fatty acid yield and the expression levels of mRNAs of
milk fat synthesis-related genes were examined. The results revealed that the hyperinsulinemiceuglycemic
clamp had no significant effect on the milk yield, the milk protein yield, the yield and
content of lactose or the yield and content of solids-not-fat (SNF) (P > 0.05). In contrast, the milk
fat percentage and milk fat yield were decreased by 35.3% and 33.6%, respectively (P C16) and 5 fatty acids synthesized de novo in the mammary gland ( 0.05), including acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), fatty acidbinding
protein (FABP), lipoprotein lipase (LPL), stearoyl-CoA desaturase (SCD) and glycerol-3-phosphate acyltransferase (GPAT). However, the expression level of the SCD gene was significantly
reduced during the post-procedure period (P