TITLE:
Investigation of Antibacterial, Cytotoxic and Antioxidant Properties of the Mangrove Plant Xylocarpus mekongensis
AUTHORS:
Mahmuda Akter, Sadia Afrin, Sk. Nazmus Sakib, Rana Biswas, Md. Morsaline Billah, Mohammad Shahedur Rahman, Umme Salma Zohora
KEYWORDS:
Xylocarpus mekongensis, Antioxidant, Antibacterial and Cytotoxicity
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.7 No.4,
April
29,
2016
ABSTRACT: The plant (Xylocarpus mekongensis) of the Sundarbans mangrove origin was evaluated for its antibacterial,
cytotoxic and antioxidant properties using methanolic and chloroformic leaf, stem and
bark extracts, respectively. The methanolic extracts contained higher amount of total phenolics,
flavonoids, tannins than the chloroformic extracts and the result was in correlation with their ferric
reducing power ability as well. However, the chloroformic bark extract contained more potent
DPPH free radical scavenging activity than others. Antibacterial activity of the extracts was determined
against both Gram-positive (Micrococcus and Staphylococcus aureus) and Gram-negative
bacteria (Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae, Salmonella typhimurium and
Salmonella paratyphi) by disc diffusion assay and their zone of inhibitions (ZOI) were measured.
Moreover, their minimum inhibitory concentrations (MIC) were determined by tube dilution
method. Chloroformic bark and stem extracts showed strong inhibition to growth of P. aeruginosa
(ZOI = 19 mm and MIC = 150 μg/ml) and S. aureus (ZOI = 19.5 mm and MIC = 250 μg/ml), respectively.
All six extracts were subjected to brine shrimp lethality bioassay for possible measure
of cytotoxicity. Concentration dependent increment in percentage mortality of brine Shrimp
nauplii produced by the extracts indicated the presence of cytotoxic principles in these extractives.
Therefore, Xylocarpus mekongensis showed antioxidant, antibacterial and cytotoxic activities.