TITLE:
Study on the Estrous Cycle Regularity of Cryopreserved Rat Ovarian Tissues after Heterotopic Transplantation
AUTHORS:
Rumana Jafarey, Syed Ali Rehan Shah Jaffri
KEYWORDS:
Transplantation, Cryopreservation, Thawing, Vaginal Smear, Ovarian Tissue, Rats
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.6 No.5,
April
26,
2016
ABSTRACT: Objective: To evaluate
the re-initiation of ovarian function in cryopreserved ovarian grafts by means
of vaginal smear of transplant rats. Methods: A total of 40 SPF-SD female rats (5
- 6 week-old) were randomly divided into three groups (blank control,
castration control and transplant group). Ovaries were removed by surgical
procedure then after cryopreservation and thawing procedures the ovarian tissues pushed inside the back muscles
gap in transplant group. On the first PO day, vaginal smear collection was
daily initiated. After 30 days, the PO day when the estrous cycle was
re-initiated was considered for analysis as well as the estrous days and the
number of estrous cycles. Results: Normal control group had a regular
estrous cycle, while the transplant group had an estrous cycle disorder within
first 2 weeks and later 2 weeks after the transplantation while the duration of
diestrus cycle lasted longer. At the same time, the castration group had lost
the normal estrous cycle, keeping continue in the stage of diestrus. Conclusion:
In transplanted animals the re-initiated ovarian function can be predicted with
alteration between estrous and diestrus phases with predominant estrous irregularity.
Moreover, short autotransplanted graft duration needs time to perfuse by new
blood vessels and hormone secretions, so could not directly affect its target
organs to function properly.