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Clinical and Laboratory Standards Institute (2002) Performance Standards for Antimicrobial Disk Susceptibility Tests: Approved Standard M2-A8. In: Performance Standards for Antimicrobial Susceptibility Testing, 8th Edition, Clinical and Laboratory Standards Institute, Wayne, 1-58.

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• TITLE: Tubastraea coccinea: A Non-Indigenous Coral (Cnidaria, Scleractinia) Collected at Arvoredo Island, South of Brazil with Potential MRSA and VRE Antimicrobial Activity

  AUTHORS: Éverson Miguel Bianco, Simone Quintana de Oliveira, Tatiana da Rosa Guimarães, Luiza Gabriela de Souza Pessoa, Maria Eduarda Alves Santos, Felipe Dal-Pizzol, Eloir Paulo Schenkel, Flávio Henrique Reginatto

  KEYWORDS: Marine Natural Products, Tubastraea coccinea, Scleractinia, Antimicrobial Activity, Methicillin- Resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Enterococcus faecalis (VRE)

  JOURNAL NAME: Open Journal of Marine Science, Vol.6 No.2, April 26, 2016

  ABSTRACT: Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coralTubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 μg/mL),Salmonella typhimurium (MIC 125 and 500 μg/mL), Escherichia coli (MIC 62.5 and 500 μg/mL) and Pseudomonas aeruginosa (MIC 62.5 and 500 μg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus(MIC 62.5 μg/mL), Klebsiella pneumoniae Carbapenemase—KPC (MIC 125 μg/mL), Methicillin-Resistant Staphylococcus aureus—MRSA (MIC 125 μg/mL) and Vancomycin-Resistant Enterococcus faecalis—VRE (MIC 62.5 μg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at Rf0.55.