TITLE:
Constitutive and Secretory Expression of the AiiA in Pichia pastoris Inhibits Amorphophallus konjac Soft Rot Disease
AUTHORS:
Jinping Wu, Zhenbiao Jiao, Fengling Guo, Leifu Chen, Zili Ding, Zhengming Qiu
KEYWORDS:
Amorphophallus konjac, Acyl Homoserine Lactonase, Pichia pastoris, Fusion-Expression
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.6 No.2,
April
5,
2016
ABSTRACT: Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) which is generated by Bacillus species has inhibitory effect on soft rot pathogen through disrupting the signal molecules (N-acylhomoserine lactones, AHL) of their Quorum Sensing system. The aim of our study is to obtain recombinant yeast which produces AiiA protein. The recombinant yeast Pichia pastoris GS115 was constructed to constitutive expression of the AiiA gene. The results of reverse transcript PCR analysis showed that the AiiA gene was expressed successfully in the yeast. Proteins extracted from YPDS showed the highest inhibition efficacy to E. carotovora compared with the other two mediums (YPD and LB) under tested conditions.