TITLE:
Transcriptional Analysis of Pseudomonas stutzeri A1501 Associated with Host Rice
AUTHORS:
Khandakar Mohiul Alam, Tao Zhang, Yonglian Yan, Wei Zhang, Min Lin, Wei Lu
KEYWORDS:
Rice, Pseudomonas stutzeri A1501, Transcriptional Analysis, Root Exudates, Colonization
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.3,
March
30,
2016
ABSTRACT: Pseudomonas stutzeriA1501,
associative and endophytic nitrogen-fixing bacterium showed the capacity of colonization
in the rice roots and considered as the good colonizer in the rice plant. The experiment
was conducted to study the expression of genes potentiality relevant to the association
of nitrogen fixing Pseudomonas stutzeri with host rice and reveal the molecular
mechanism by which underlying interaction between bacteria and host rice. The bacteria
were shown to be uniformly distributed on the rhizoplane of the root and the density
of bacteria was found at the intercellular junction and micro colony developed on
the surface of the epidermal cells and on the cellular junctions. Root exudates
of rice were the major components of carbon and energy sources for bacteria. RT-PCR
analyses of pilK, metE, rpoN and fdhE genes expression of P. stutzeri A1501 were performed at positive
and negative (control) conditions. After 1 h, it was found that pilK, metE and rpoN transcription
were increased 5.7, 6.4 and 3.4-fold, respectively, whereas in the fdhE gene has no expression. Consequently,
after 4 h pilk, fdhE, metE and rpoN were decreased -1.9, -4.4, -0.2 and -0.8-fold, respectively. The gene pilK, expression was up-regulation after
1 h and down-regulation after 4 h that has twitching motility to convey the bacterial
cell to point of attachment in to host plant. The gene expressions of the bacteria, pilK, metE, rpoN and fdhE were up- and down-regulated during the
influence of root exudates which regulated the colonization of bacteria during plant-microbe
interaction.