TITLE:
First Report on the Presence of Phyllachora sp. in Corn Crops at Toluca, Estado de Mexico
AUTHORS:
Erika Natalia Ríos-Herrera, Laila Pamela Partida-Martínez, Yisa María Ochoa-Fuentes, Ernesto Cerna-Chávez, Francisco Daniel Hernández-Castillo, Alberto Flores-Olivas, Víctor Olalde-Portugal, Raúl Rodríguez-Guerra
KEYWORDS:
Phyllachora maydis, Tar Spot Complex, ITS
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.5,
March
30,
2016
ABSTRACT: Symptoms of Tar Spot Complex (TSC) in corn appear in the form of black spots surrounded by a chlorotic halo known as fish-eye. The crop with these symptoms was found at San Pablo Autopan, Estado de Mexico. The stromal region of foliar lesions was cut longitudinally, showing perithecia, asci and ascospores typical of Phyllachora maydis. The results were confirmed by PCR-ITS with ITS 1 and ITS4 initiators, cloned by pJET, before being sequenced and compared against NCBI’s data base. Phyllachora identity was confirmed. This is the first report where TSC pathogen has been detected under conditions that had not been previously reported, for instance, at 2600 meters above the sea level.