TITLE:
The Effect of Cangfudaotan Tang on Expression of Organic Anion Transporting Polypeptide (oatp4a1) in Rat Ovary and Uterus Tissues of Obese PCOS
AUTHORS:
Huachao Mai, Jianjun Li, Kefang Chen, Xiangping Hou, Aizhen Pan
KEYWORDS:
Cangfudaotan Tang, Polycysticovarian Syndrome, oatp4a1, Sex Hormone
JOURNAL NAME:
Chinese Medicine,
Vol.7 No.1,
March
23,
2016
ABSTRACT: Objective: The objective is to explore the effect of Cangfudaotan Tang on expression of organic anion transporting polypeptide (oatp4a1) in ovary and uterus tissues in rats with obese polycystic ovary syndrome. Methods: Poresky method and high fat diet were used to build obese PCOS rat models. The PCOS rat models were randomized into model control group, metformin group, the low dose Cangfudaotan Tang group and the high dose Cangfudaotan Tang group with 10 rats in each group. The metformin group was treated with metformin 43 mg/100g intragastric administration for two weeks, and the high dose Cangfudaotan Tang group and the low dose Cangfudaotan Tang group were given Cangfudaotan Tang 5.68 g/kg/d and 1.42 g/kg/d intragastric administration respectively for two weeks. Then the rats were anesthetized. Each rat was taken ovary and uterus tissues, and the expression of oatp4a1 Protein was detected in the tissues by using Western Blot techniques. The level of sex hormones as testosterone (T), follicle stimulating hormone (FSH), estradiol (E2), and luteinizing hormone (LH) and insulin resistance were detected by ELLISA techniques. Results: The expression of oatp4a1 in rat ovary and uterus tissue of model group was down-regulated compared to blank control group (p ≤ 0.05). The expression of oatp4a1 in ovary and uterus tissue of treatment group was up-regulated compared to model group (p