TITLE:
The Function of miR-31 Expression in Differentiation of Neural Stem Cells
AUTHORS:
Xiao Li, Yuantao Gao, Pengfei Li, Feng Tian, Jing Wang, Chunfang Wang
KEYWORDS:
Spinal Cord Injury, microRNA, Neural Stem Cells, Transplantation, Mouse
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.4 No.3,
March
17,
2016
ABSTRACT: Spinal cord injury (SCI) is a severe
complication of acute spinal injury, it can lead to axonal degeneration and necrosis
of neurons, causing damage cross section below the movement, then loss of sensory,
reflex and autonomous control function below the damage section. Nowadays, there
is increasing evidence that transplantation of neural stem cells can help to repair
spinal cord injury after spinal cord injury. The aim of this study was to research
the function of miR-31 expression in differentiation of neural stem cells. Mir-31
was transfected the mimics and inhibitor into neural stem cells. By the morphological
observation, the cell over expression of miR-31 was closer to NSCs. With the RT-PCR,
Hb9 and STMN1 were up-regulate when miR-31 was inhibited, and Nestin was down-regulate.
When miR-31 was over expression, the expression of Nestin and Hb9 was up-regulate.
These findings suggest that miR-31 may play a significant role in proliferation
and differentiation of neural stem cell and are potential targets for therapeutic
interventions following spinal cord injury.