TITLE:
Properties of Bacillus species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate
AUTHORS:
Frank Anayo Orji, Ekaette Nduka Dike, Adekunle Kolawole Lawal, Abdulateef Omoniyi Sadiq, Yewande Suberu, Ariyo Caleb Famotemi, Agatha Isioma Ugbana, Folake Fashola, Blossom Ita, Samuel Olakiitan Olatope, Emoleila Ejiya Itoandoan, Abimbola Olajumoke Adefiranye, Gloria Nwakaegho Elemo
KEYWORDS:
Cellulase, Bacillus species, Crude Activities, Temperature, pH Tolerance, Brewers Spent Grains (BSG)
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.7 No.3,
March
16,
2016
ABSTRACT: The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on
supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity
between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and
crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of
carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223
cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The
co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria
using local raw materials such as Brewers Spent Grain reduces enzyme cost; cost of finished
products and increases gross domestic products.