TITLE:
Validated UPLC/Q-TOF-MS Method for Determination of Poliumoside in Rat Plasma and Its Application to Pharmacokinetic Study
AUTHORS:
Hao Qian, Danyi Lu, Wan Li, Xiaotong Zhou, Baojian Wu, Zhiguo Ma
KEYWORDS:
Poliumoside, UPLC/Q-TOF-MS, Rat Plasma, Pharmacokinetics
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.7 No.3,
March
15,
2016
ABSTRACT: Poliumoside is the
main active constituent of Callicarpa
kwangtungensis Chun (CK), a traditional Chinese medicine for management of
hemostasis. In this study, a rapid and selective ultra-performance liquid chromatography coupled with
quadrupole time-of-flight mass spectrometry (UPLC/ Q-TOF-MS) method was
developed and validated to quantify poliumoside in rat plasma. The targeted
analytes in rat plasma were prepared through protein-precipitation method using
10% trichloroacetic acid (TCA). The chromatographic separation was performed on a Waters BEH C18 column (2.1 × 100 mm, 1.7 μm) by acetonitrile-water containing 0.1% formic
acid. The calibration curve was linear over the range of 50 - 10,000 ng/mL (r2 > 0.99). The intra-day or inter-day precision was less than 7.97% and
accuracy was within ?7.00% - 3.36%. The developed method was successfully
applied to pharmacokinetic study of poliumoside in rat plasma. Although being
rapidly absorbed (Tmax ≤ 30 min), poliumoside was poorly bioavailable
after oral administration (the absolute bioavailability was only 0.69%).