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Liu, M.Z., Zhang, Y.L., Zeng, M.Z., He, F.Z., Luo, Z.Y., Luo, J.Q., Wen, J.G., Chen, X.P., Zhou, H.H. and Zhang, W. (2015) Pharmacogenomics and Herb-Drug Interactions: Merge of Future and Tradition. Evidence-Based Complementary and Alternative Medicine, 2015, Article ID: 321091.
http://dx.doi.org/10.1155/2015/321091

has been cited by the following article:

• TITLE: Validated UPLC/Q-TOF-MS Method for Determination of Poliumoside in Rat Plasma and Its Application to Pharmacokinetic Study

  AUTHORS: Hao Qian, Danyi Lu, Wan Li, Xiaotong Zhou, Baojian Wu, Zhiguo Ma

  KEYWORDS: Poliumoside, UPLC/Q-TOF-MS, Rat Plasma, Pharmacokinetics

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.7 No.3, March 15, 2016

  ABSTRACT: Poliumoside is the main active constituent of Callicarpa kwangtungensis Chun (CK), a traditional Chinese medicine for management of hemostasis. In this study, a rapid and selective ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/ Q-TOF-MS) method was developed and validated to quantify poliumoside in rat plasma. The targeted analytes in rat plasma were prepared through protein-precipitation method using 10% trichloroacetic acid (TCA). The chromatographic separation was performed on a Waters BEH C18 column (2.1 × 100 mm, 1.7 μm) by acetonitrile-water containing 0.1% formic acid. The calibration curve was linear over the range of 50 - 10,000 ng/mL (r2 > 0.99). The intra-day or inter-day precision was less than 7.97% and accuracy was within ?7.00% - 3.36%. The developed method was successfully applied to pharmacokinetic study of poliumoside in rat plasma. Although being rapidly absorbed (Tmax ≤ 30 min), poliumoside was poorly bioavailable after oral administration (the absolute bioavailability was only 0.69%).