TITLE:
Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts
AUTHORS:
Rehab Mobark Osman Mohammed, Saad Mohmmed Hussein Ayoub
KEYWORDS:
C. aurantifolia, Antimicrobial Activity, Limone, Phytochemical Screening, Liquid Solid Column Chromatographic
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.7 No.3,
March
15,
2016
ABSTRACT: Citrus aurantifolia, the family Rutaceae,
which consists of 150 genera and 900 species, is gaining grounds as important source
for treatment in complementary medicine, and commonly called limone. Chloroform,
methanol and ethanol extract exhibited significant antimicrobial activity and highlighted
the biological monitoring of activity from the methanol extract of seeds. Phytochemical
screening of the diethyl ether, methanol and aqueous extracts of seeds confirmed
the presence of alkaloids, saponins, sterols and triterpenes, carotenoids, coumarins,
tannins and carbohydrates. Chloroform extract was fractionated by sing liquid solid
column chromatographic technique and column fractions eluted with chloroform and
methanol. The antibacterial secondary metabolites were achieved by fractionation
of the active. The seed extracts were monitored analytically by TLC preparative
and some chemical analyses of seeds have been done such as determination of ash,
nitrogen, protein content.