TITLE:
Studies on Antibiotic Sensitivity Pattern of Pseudomonas aeruginosa Isolated from Hospitalized Patients
AUTHORS:
Shahidul A. Chowdhury, Jabun Naher, Abdullah A. Mamun, Ruhul A. Khan, Jannatul Ferdous, Sharmin Sultana
KEYWORDS:
Antibiotic, Sensitivity, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration), Pseudomonas aeruginosa
JOURNAL NAME:
Open Access Library Journal,
Vol.1 No.6,
September
25,
2014
ABSTRACT: This study was performed to evaluate the antibiotic sensitivity pattern and determination of MIC (Minimum inhibitory concentration) and MBC (Minimum bactericidal concentration) value of Pseudomonas aeruginosa. The samples were collected from hospitalized and outdoor patients of Chittagong Maa-O-Shisu General hospital (October, 2010 to April, 2011). From the sample (blood, pus and urine) the isolate was identified on the basis of their morphological, cultural and biochemical characteristics. Antibiotic sensitivity pattern of the isolates was then observed (Disc diffusion method). It was found that Ciprofloxacin, Azithromycin and Chloramphenicol showed the highest percentages of sensitivity (above 60%) so they were the most effective drug for the treatment of Pseudomonas aeruginosa infection, where Vancomycin, Amphicillin and Amoxicillin showed the lowest percentages of sensitivity as well as less effective drug. Minimum Inhibitory Concentration (MIC) (Turbidimetric Method) values were determined against six antibiotics (Ciprofloxacin, Azithromycin, Kanamycin, Erythromycin, Chloramphenicol and Gentamycin). The MIC of Ciprofloxacin and Azithromycin for the majority of clinical isolates was 2 to >64 μg/ml. Like that the MIC of Gentamycin, Kanamycin and Chloramphenicol for the clinical isolates was 4 to >64 μg/ml. MIC value of Erythromycin was between 8 to >64 μg. The Minimum bactericidal concentration (MBC) for all these six drugs were also done and the result was between 2 to >64 μg/ml.