TITLE:
Digestion of Mitochondrial DNA of Some Sudanese Camel Breeds Using Three Different Restriction Enzymes
AUTHORS:
M. Sulieman, Yossra A. Malik, S. Sid Ahmed, A. A. Makkawi, Nahid Gornas
KEYWORDS:
Mitochondrial DNA, Camel, Sudan
JOURNAL NAME:
Open Access Library Journal,
Vol.1 No.4,
July
14,
2014
ABSTRACT: The purpose of this study was to estimate relationship between and within some Sudanese camel breeds (Kababeish, Shanabla and Nyalawei) by digestion of mitochondrial DNA using three different restriction enzymes. DNA was extracted from 45 blood samples of Sudanese camels (15 form each breed). Polymerase chain reaction was done using specific primers in order to amplify the D-Loop region. The PCR products were digested using 3 different restriction enzymes (tag1, hinf and scal). The products were run on agarose gel 2%. The result of this study revealed differences between the three breeds according to digested and undigested samples. Tag1 did not cut any of the 45 samples of all breeds, while scal cut 4.4% of the Shanabla breed, 6.7% of Kababeish breed, but did not cut any of Nyalawei camels. The third restriction enzyme hinf cut all sample from Shanabla camels, and also cut 95.6% of Kababeish breed and also 95.6% of Nyalawei camels.