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Sun, Z.J., Xia, S.D., Feng, S.M., Zhang, Z.K., Rahman, M.M., Rajkumar, M. and Jiang, S.G. (2015) Effects of Water Temperature on Survival, Growth, Digestiveenzyme Activities, and Body Composition of the Leopard Coral Grouper Plectropomus leopardus. Fisheries Science, 81, 107-112.
http://dx.doi.org/10.1007/s12562-014-0832-9

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• TITLE: Optima of Trypsin-Catalyzed Hydrolysis and Its Inhibition Determined by SDS-PAGE

  AUTHORS: Xueke Zhou, Tingting Wang, Anjun Wang, Renqiang Li

  KEYWORDS: Trypsin-Catalyzed Hydrolysis, Trypsin Inhibitor, Optimum Condition, SDS-PAGE, Assay Method

  JOURNAL NAME: Advances in Enzyme Research, Vol.4 No.1, March 4, 2016

  ABSTRACT: SDS-PAGE was applied to determine trypsin activity and inhibition. After the hydrolysis by trypsin to substrate bovine serum albulnin (BSA) under different temperatures and pH, the hydrolysis degree of BSA was conducted using SDS-PAGE. From the quantitative analysis to the electrophoresis bands of BSA and its hydrolysis products in SDS-PAGE pattern, the change of trypsin activity was determined, and then the optimum temperature at 40°C and the optimum pH at pH 8.5 - 8.7 for trypsin activity were obtained. All the target bonds in BSA molecule could be hydrolyzed at the same time by trypsin. The inhibition was due to the binding of inhibitor to trypsin, which made it impossible for trypsin to touch the substrate protein. SDS-PAGE was demonstrated to be also an effect method for assaying the characteristics of trypsin activity and its inhibition.