Article citationsMore>>

Vanden Branden, M., Wrighton, S.A., Ekins, S., Gillespie, J.S., Binkley, S.N., Ring, B.J., Gadberry, M.G., Mullins, D.C., Strom, S.C. and Jensen, C.B. (1998) Alterations of the Catalytic Activities of Drug-Metabolizing Enzymes in Cultures of Human Liver Slices. Drug Metabolism and Disposition, 26, 1063-1068.

has been cited by the following article:

• TITLE: Application of Precision-Cut Rat Liver Slice to Study the Influence of Monocrotaline, Tussilago farfara Alkaloids on the Expression of Cytochrome P450 Enzymes

  AUTHORS: Hailin Wang, Lianqiang Hui, Chun Li, Ting Liu, Chang’an Yu, Chunyu Cao, Ran Hao, Yi Zhang

  KEYWORDS: Precision-Cut Liver Slices, Monocrotaline, Tussilago farfara Alkaloids, Hepatotoxity, Cytochrome P450 Enzymes

  JOURNAL NAME: Health, Vol.8 No.4, February 29, 2016

  ABSTRACT: Precision-cut liver slice has been successfully used to study the mechanism of drug-induced hepatotoxicity, the prediction of liver toxicity, the discovery of early hepatic toxicity biomarker and the metabolism of drug in liver. We detected the expression of CYP3A4, CYP2B1 + CYP2B2 and CYP2E1 in precision-cut liver slice after co-cultured with monocrotaline or Tussilago farfara alkaloids to investigate the hepatotoxicity mechanism of those drugs. After co-culturing with monocrotaline or Tussilago farfara alkaloids for 6 hours, the expression of CYP3A4 in the microsome of precision-cut liver slices was detected by Western blot, and the expressions of CYP2B1 + CYP2B2 and CYP2E1 were detected by immunofluorescence. The results showed that monocrotaline induced the expression of CYP3A4 and CYP2B1 + CYP2B2, and Tussilago farfara alkaloids obviously up-regulated the expression of CYP2E1 and CYP3A4. Thus, we conclude that the up-regulation of CYP3A4, CYP2B1 + CYP2B2 and CYP2E1 may be one of the toxic mechanisms of liver injury of those drugs.