TITLE:
Evaluation of Estrogenic Receptor (ER) and Aryl Hydrocarbon Receptor (AhR) Agonists in Three Gorges Reservoir, China Using SPMD-Based Virtual Organisms
AUTHORS:
Jingxian Wang, Bernhard Henkelmann, Toine F. H. Bovee, Gerd Pfister, Liang Zhang, Karl-Werner Schramm
KEYWORDS:
ER Activity, EROD Activity, Three Gorges Reservoir, Virtual Organism, Semipermeable Membrane Device
JOURNAL NAME:
Journal of Environmental Protection,
Vol.7 No.3,
February
26,
2016
ABSTRACT: The evaluation of estrogenic activities and
aryl hydrocarbon receptor (AhR) agonists in water from Three Gorges Reservoir
(TGR) China was conducted by in vitro bioassays combined with SPMD-based
virtual organisms (VO). VOs were deployed at seven sites in the Three Gorges
Reservoir (TGR), China for two periods in 2009. The estrogenic activity was
assessed using a rapid yeast estrogen bioassay, based on the expression of a
green fluorescent reporter protein (yEGFP). The AhR activity was detected
employing rat hepatoma cell line (H4IIE). The results indicate that AhR
agonists distributed widely in water of TGR and almost homogenously distributed
in most area of TGR. Weak antiestrogenic activities were also found
homogenously distributed in water of TGR. Further studies are needed to
determine the identities of these estrogenic compounds and AhR agonists and
their potential adverse effects on wild biota in TGR.