TITLE:
Comparison of Blood Group Molecular Genotyping to Traditional Serological Phenotyping in Patients with Chronic or Recent Blood Transfusion
AUTHORS:
Zhan Ye, Daoping Zhang, Leonard Boral, Counts Liz, John May
KEYWORDS:
Blood Group, Molecular Genotyping, Serological Phenotyping, Chronic Blood Transfusion
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.4 No.3,
February
26,
2016
ABSTRACT: Objectives: Accurately identifying the
Antigens (Ags) on recipient red blood cells (RBCs) is critical in prevention of
RBC alloimmunization in chronically transfused patients. The goal of this study
was to compare RBC molecular genotyping to serological phenotyping in those
patients. Methods: Serological phenotyping and molecular genotyping methods
were used to study blood samples from 18 healthy blood donors and 16 transfused
patients. Reticulocyte harvesting or hypotonic cell separation was added to
recheck RBC phenotypes of the patients with discrepancies between phenotyping
and genotyping. Results: No discrepancies were found between the two genotyping
methods in all the donors and patients. 1 of 9 sickle-cell disease (SCD) patients
and all 3 thalassemia patients demonstrated discrepancies in multiple blood
groups between phenotyping and genotyping, which were not corrected by
reticulocyte harvesting or hypotonic cell separation. Conclusions: These
findings suggest that RBC molecular genotyping is superior to serological phenotyping
in chronically transfused SCD or thalassemia patients.