TITLE:
Model of Sterile Intestine of Mices with Normal Microfloras: Histological and Ultrastructural Visualization Formation Kyst Organ-Like Intestine for the Study of Interaction of Pathogen Agents with the Host Sterile Intestine
AUTHORS:
Karlen Hovnanyan
KEYWORDS:
Sterile Intestine, Model, Electron Microscopy, Ultrastructura, Parasit-Host
JOURNAL NAME:
Open Access Library Journal,
Vol.1 No.2,
May
29,
2014
ABSTRACT: For obtainment of the isolated sterile intestine, inbred lines of mice C3HA, CBA, C57BL/6, BalB/c are used at same time; it is carried out transplantation of small and large intestines of embryons corresponding lines of mice subcutaneously in adult singen mice. In capacite of test objects we make use of cultures of Salmonella typhimurium. The side of organ like kysts of isolated sterile of small and large intestines’ models on the histological and transmission electron-microscopic (TEM) preparations have their own typical composition. In case of electron-microscopic examination of steril small intestine they were revealed microvillus of epithelium, Salmonella typhimurium in lining epithelium and phagosoma of lymphoid cells of lamina propria of mucose.