TITLE:
Sensitivity and Specificity of Various Serological Tests for Detection of Brucella spp. Infection in Male Goats and Sheep
AUTHORS:
Waffa A. Ahmed, Shaimaa A. Majeed, Ameer H. Abdul Ameer, Nawal D. Mahmmod, Nameer I. Saeed, Luma. Y. Hanaa
KEYWORDS:
Brucellosis, RBPT, ICA, iELISA, Sheep, Goat
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.2,
February
24,
2016
ABSTRACT: Brucellosis
is a zoonosis found throughout the world with major implications both in the
field of public health and farming economy. It is most often caused by Brucella abortus and Brucella melitensis. This study aimed for evaluation of the
sensitivity and specificity of immunochromatographic assay (ICA) and comparing the
results with those obtained from Rose Bengal Plate Test (RBPT) and in direct
Enzyme Linked Immunsorbent Assay (ELISA) for serodiagnosis of sheep and goat
brucellosis. Out of 41 sera samples (21 ram and 20 male goats) which were
examined, antibodies against Brucella were detected in ram 8 (38.09%), 6
(28.57%) and 16 (76.19%) in ram samples, whereas they were detected in 3 (15%),
1 (5%) and 12 (60%) serum sample of goats males by RBPT, (ICA) and iELISA
respectively. The kappa value between iELISA and ICA was -0.08 and that between
RBPT and ELISA was -0.01 in ram and the kappa value between iELISA and ICA was -0.06
and that between RBPT and ELISA was -0.14 in male goats. The sensitivity for
RBPT and ICA were 37.5% and 25% while specificity was 60% and 60% in ram; whereas,
in male goats, sensitivity for RBPT was 8.33% and ICA was 8.33% while
specificity was 75% and 100% respectively. It can be concluded that the
developed ICA is immunodiagnostic assay, and it is rapid, non-expensive,
economical and suitable for large-scale screening in developing countries and rural
areas.