TITLE:
Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops
AUTHORS:
Ahmad Hama Ameen Rashid, Djshwar Dhahir Lateef
KEYWORDS:
T-DNA, Particle Bombardment, MSNs, Silicon Carbide-Mediated Transformation, Electroporation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.1,
January
28,
2016
ABSTRACT: From the early past to the present, biotechnologies have produced the ability to genetically transform a wide variety of plant species. The plant transformation technologies have changed the face of agriculture and plant biology. Plant genetic transformation is one of the key technologies for crop improvement in addition to emerging approach for producing recombinant proteins in plants. Both plastid genomes and plant nuclear can be genetically modified. Until now, essential functional differences between the prokaryotic-like genome of the plastid and the eukaryotic genome of the plant cell nucleus will have an impact on characteristics of transgenic organism. Thus, the main goals are to generate transgenic plants with the traits of interest as well as minimizing the amount of transgenic DNA in plants while maximizing stability of gene expression and trait performance. In this review, two broad groups of gene delivery methods will be discussed namely, (bilogical and physical methods) and subsequently there applications for improving disease resistance will be discussed.