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Neves-Petersen, M.T., Gryczynski, Z., Lakowicz, J., Fojan, P., Pedersen, S., Petersen, E. and Petersen, S.B. (2002) High Probability of Disrupting a Disulphide Bridge Mediated by an Endogenous Excited Tryptophan Residue. Protein Science, 11, 588-600.
http://dx.doi.org/10.1110/ps.06002

has been cited by the following article:

• TITLE: Changes in Human Hair Induced by UV- and Gamma Irradiation

  AUTHORS: Ervin Palma, David Gomez, Eugene Galicia, Viktor Stolc, Yuri Griko

  KEYWORDS: Hair, Fluorescence, Radiation, Chemical Integrity

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.7 No.1, January 28, 2016

  ABSTRACT: The effect of UV- and 137Cs gamma radiation on the structural and chemical integrity of human hair was studied to determine the feasibility of using human hair as a non-invasive biomarker of radiation exposure to ionized gamma- and non-ionized UV-radiation. Steady state tryptophan (Trp) fluorescence and chemical analytical methods were used to evaluate the molecular integrity of Trp fluorophores and SH-groups in hair proteins and to assess the radiation induced damage quantitatively. It was found that human hair fibers were progressively damaged by exposure to both UV- and ionized gamma radiation. Damage to the hair was evidenced by a decrease in the fluorescence intensity as a result of observed depletion of the amino acid tryptophan as well as significant reduction in a number of free SH-groups in hair proteins. Hair damage was dose-dependent for exposures between 0 and 10.0 Gy and 0 - 20 J/cm2 of UV-radiation. Additional results demonstrate that hair-fibers exposed to gamma rays, with much higher quantum energy than UV, undergo a smaller extent of changes in Trp fluorescence than when exposed to lower or equal energy of UV-irradiation. The stable Trp fluorophore appears to be extremely sensitive to UV-radiation in contrast to the ionized gamma radiation whose damage is originated from the reaction of free radicals and direct deposition of energy. We conclude that fluorescence spectroscopy represents a useful tool in the quantitative evaluation of the radiation exposure and could also be used for the rapid and non-invasive assessment of radiation dose i.e. biodosimeter. The approach is simple, non-invasive and appears to have considerable potential that enables quantitative evaluation of radiation dose exposure in a single hair fiber.