TITLE:
Urinary Tract Infection Escherichia coli Is Related to the Environmental Escherichia coli in Their DNA Barcoding and Antibiotic Resistance Patterns in Grenada
AUTHORS:
Karla Farmer, Abi James, Ravindra Naraine, Grace Dolphin, Wayne Sylvester, Victor Amadi, Svetlana V. Kotelnikova
KEYWORDS:
Exogenous DNA, E. coli, Urinary Tract Infection, UPEC, (GTG)5
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.1,
January
26,
2016
ABSTRACT: Urinary Tract Infections (UTIs) are among one of the most common infections
in women, with uropathogenic E. coli (UPEC) being involved in 80% of cases.
In addition, E. coli exhibits an increasing resistance to broad spectrum antimicrobial agents as
well as the subsequent generations of these drugs. The genetic diversity and antibiotic
resistance patterns of both clinical and environmental E. coli isolates were studied to predict the potential of transmission
of organisms and genes for antibiotic resistance from three different major eco-habitats including
the gut of iguanas’ fresh and marine waters
and the human urinary tract. (GTG)5 and BOX-PCR extragenic DNA fingerprinting
allowed for the tracking of the relatedness of four different ecotype groups. Both
DNA fingerprinting methods targeted non-protein coding or exogenous palindromic
DNA and demonstrated shared origin and intraspecies level of genomic diversity within
the population of the studied bacteria. DNA fingerprinting based on BOX-PCR was
less discriminating than the (GTG)5-PCR, and produced five major clades.
BOX-PCR analysis indicated that 44% of the UTI E. coli isolates was comprised within a single clade, therefore it correlated
better with ecotype distribution. The (GTG)5 PCR based co-clustering
analysis showed that the clinical isolates appeared to have a closer relationship
to iguana E. coli isolates than to the
fresh and marine water isolates. However, in accordance with the BOX PCR co-clustering
analysis, the clinical isolates were most similar to the marine water isolates,
followed by the freshwater and iguana E. coli isolates. Seventy two percent of antibiotic resistance patterns were shared by the
UTI strains with E. coli isolated from
freshwater, followed by iguana.