TITLE:
The Profile of Secondary Metabolites of Sponge Clathria reinwardtii Extract as a Result of Fe Accumulation in Spermonde Archipelago
AUTHORS:
Lydia Melawaty, Kristiana Pasau
KEYWORDS:
Clathria reinwardtii, Fe, GC-MS, FTIR
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.5 No.7,
December
30,
2015
ABSTRACT: Study on the effect of Fe metal concentrations in Spermonde Archipelago on secondary metabolites production from sponge was conducted. The percent of peak area and m/z from GC-MS at tR = 13.555 were affected by accumulated Fe in Chlathria reinwardtii, where at high Fe concentration a lower percent peak area was produced compared to Clathria rein-wardtii extract at low Fe concentration. Chromatogram CR-LL-DCM produced 2 peaks at tR = 21.401 and 25.174 which were not observed in CR-BC-DCM chromatogram. This indicated that Clathria reinwardtii in Lae-Lae island produced metabolite secondary compounds that were not produced by Clahtria reinwardtii in Barrang Caddi island. FT-IR analysis indicated that Clathria reinwardtii accumulating Fe with highest and lowest concentration produced strong peaks at wavenumber of about 3400 cm-1 for O-H and N-H stretches. O-H pliable and C-O stretch peaks at wavenumber of about 1200 cm-1 had changes from medium intensity at low Fe concentration (CR-BC-DCM) to weak at high Fe concentration (CR-LL-DCM).