TITLE:
Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health
AUTHORS:
Dalia A. Hamza, Sohad M. Dorgham, Amany Arafa
KEYWORDS:
Coagulase, coa Gene, mecA Gene, Staphylococci, Methicillin
JOURNAL NAME:
Advances in Infectious Diseases,
Vol.5 No.4,
December
25,
2015
ABSTRACT: Emerging antimicrobial
resistance among CNS is a concern in veterinary and human medicine. Coagulase
test is considered as the key test to differentiate staphylococci to two
groups, coagulase positive staphylococci (CPS) and coagulase negative
staphylococci (CNS). A total of 200Staphylococcistrains were isolated with
percentage 66.7% (200/300) from quarter milk samples. The total ofS. aureusstrains are 70 with percentage 35%
(70/200). Among 70 strains ofS.
aureus, 30 strains are coagulase positiveS.
aureuswith percentage 43%
(30/70) and coagulase negativeS.
aureus57% (40/70). CNS other
thanS. aureuswas detected with percentage 65%
(130/200) from subclinical mastitic cows. We examine sixty isolates of
staphylococci recovered from subclinical mastitis in dairy cattle which divided
as ten isolates of coagulase positiveS.
aureus(CPS. aureus), ten isolates
of coagulase negativeS.
aureus(CNS. aureus)
and forty isolates of coagulase negative staphylococci (CNS) which identified
using API-Staph Kits asS.
chromogenes,S.
simulans,S.
haemolyticus,S.
epidermidisandS. cohnii.The genotypic detection ofcoagene andmecA gene was screened in CPS. aureus, CNS. aureusand CNS.