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T. Cedervall, I. Lynch, S. Lindman, T. Berggard, E. Thulin, H. Nilsson, K.A. Dawson, and S. Linse, “Understanding the Nanoparticle-protein Corona using Methods to Quantify Exchange Rates and Affinities of Proteins for Nanoparticles,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 7, 2007, pp. 2050-2055.

has been cited by the following article:

• TITLE: Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles

  AUTHORS: Martin Lundqvist, Tord Berggård, Erik Hellstrand, Iseult Lynch, Kenneth A. Dawson, Sara Linse, Tommy Cedervall

  KEYWORDS: Apolipoprotein A-I, nanoparticles, selective purification

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.2 No.3, May 20, 2011

  ABSTRACT: Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.