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T. Cedervall, I. Lynch, M. Foy, T. Bergg?rd, S.C. Donnelly, G. Cagney, S. Linse, and K.A. Dawson, “Detailed Identification of Plasma Proteins Adsorbed on Copolymer Nanoparticles,” Angewandte Chemie International Edition, Vol. 46, No. 30, 2007, pp. 5754-5756.

has been cited by the following article:

• TITLE: Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles

  AUTHORS: Martin Lundqvist, Tord Berggård, Erik Hellstrand, Iseult Lynch, Kenneth A. Dawson, Sara Linse, Tommy Cedervall

  KEYWORDS: Apolipoprotein A-I, nanoparticles, selective purification

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.2 No.3, May 20, 2011

  ABSTRACT: Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.