TITLE:
Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with Piper betle L. Leaves
AUTHORS:
Ashwini Kumar Dubey, Kadirvelu Jeevaratnam
KEYWORDS:
Acid Tolerance, Adhesion, Immune Modulation, Lactobacilli, Probiotic
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.13,
December
18,
2015
ABSTRACT: The in vitro screening of lactobacilli
isolates from uttapam batter, fermented supplementing with Piper betle L. leaves, was performed in order to select potent
isolates for probiotic use. Their resistance to simulated gastric and
intestinal juices as well as their adhesion to epithelial intestinal HCT-15 and
vaginal HeLa cell lines were assessed and also evaluated for their
immunomodulatory ability in ex vivo condition. Isolates were able to adhere as well as reduce pathogen adhesion to
monolayer cell lines’ surfaces and reduced production of pro-inflammatory
cytokine TNF-α in LPS treated PBMCs,
while enhanced that of anti-inflammatory cytokine IL-10. The isolates exhibited
properties of auto-aggregation, co-aggregation, hydrophobicity, bile salt
hydrolase activity and strong antimicrobial activity against pathogenic
bacteria. Thus, the different lactobacilli isolates displayed potent probiotic
and immunomodulatory properties among which AJ7 and AJ82 had a great potential
and may have applications in fermented foods as immunomodulatory probiotic
additives.