TITLE:
Determination of Tea Saponin in Camellia Seed Oil with UV and HPLC Analysis
AUTHORS:
Hadeil Omer Abdelgadir Ahmed, Chengming Wang
KEYWORDS:
Tea Seed Oil, Saponification, UV, High Performance Liquid Chromatography (HPLC)
JOURNAL NAME:
World Journal of Engineering and Technology,
Vol.3 No.4C,
December
17,
2015
ABSTRACT: A simple procedure is described for the HPLC
and UV determination of tea saponin in tea seed oil. Determinate was accomplished
with UV wavelength detection 550 nm for saponification sample, and HPLC was done
under conditions: C18 analytical column of TC-C18, 4. 6 × 250 mm, column temperature
at room temperature, injected sample volume was 10 μL, mobile phase’s methanol,
flow-rate 0.8 ml/min and detection wavelength 280 nm.