TITLE:
Determination of the Genetic Structure of the Oleaginous Lagenaria siceraria of the Nangui Abrogoua University Germplasm Collection
AUTHORS:
Ahou Anique Gbotto, Kouamé Kevin Koffi, Jean-Pierre Baudoin, Irié Arsène Zoro Bi
KEYWORDS:
Genetic Structure, Lagenaria siceraria, Null Alleles, Oleaginous, SSR
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.6 No.19,
December
17,
2015
ABSTRACT: Thirty accessions of Lagenaria siceraria from the Nangui Abrogoua University germplasm collection
were analyzed using three microsatellite markers. The average Polymorphism Information
Content (PIC) value was 0.61. The average observed heterozygosity value (H0 = 0.631) compared
to the average expected heterozygosity value (He = 0.645) did not show significant differences in
the selected accessions, which suggested random mating in the set of accessions. Within accession
inbreeding estimate (FIS), was 40% and was not significantly different from zero. The reduction of
heterozygotes was likely the result of presence of null alleles. Analysis of Molecular Variance
(AMOVA) within and among 30 accessions of L. siceraria revealed that 39% of the total variation
resides among accessions and 61% within accessions. Accession structuring pattern derived from
Bayesian clustering analysis revealed two clusters. Based on the genetic structure of the accessions
analyzed, a sampling strategy to collect and conserve genetic resources of L. siceraria was
suggested.