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Vinje, J., Vennema, H., Maunula, L., von Bonsdorff, C.H., Hoehne, M., Schreier, E., Richards, A., Green, J., Brown, D., Beard, S.S., Monroe, S.S., de Bruin, E., Svensson, L. and Koopmans, M.P. (2003) International Collaborative Study to Compare Reverse Transcriptase PCR Assays for Detection and Genotyping of Noroviruses. Journal of Clinical Microbiology, 41, 1423-1433.
http://www.ncbi.nlm.nih.gov/pubmed/12682125
http://dx.doi.org/10.1128/jcm.41.4.1423-1433.2003

has been cited by the following article:

• TITLE: Acute Diarrhea Etiology in Young Children and Adults in the Republic of Maldives—A Point Prevalence Study

  AUTHORS: Abdul A. Yoosuf, Ladaporn Bodhidatta, Siriporn Sornsakrin, Fathimath I. Manik, Shah Saeed, Mohamed Hassan, Carl J. Mason

  KEYWORDS: Diarrhea, Maldives, Rotavirus, Norovirus

  JOURNAL NAME: Open Journal of Medical Microbiology, Vol.5 No.4, December 4, 2015

  ABSTRACT: Introduction: Despite its recent status of middle-income country in WHO’s South-East Asia Region, diarrhea remains an important yet unresearched public health issue in the Republic of Maldives. Methodology: We conducted a one-month cross-sectional study in children and adults with acute diarrhea at three regional hospitals in Maldives in August-September 2007 to investigate the point-prevalence of diarrhea etiologic agents. Enteric Bacteria was identified by a standard microbiology technique and isolates were submitted for antimicrobial susceptibility testing. Rotavirus, astrovirus and adenovirus were detected by enzyme-linked immunosorbent assays (ELISA). Real-time reverse-transcription polymerase chain reaction (RT-PCR) was used to test for norovirus. Results: We enrolled 73 children and 57 adults with acute diarrhea. The most common pathogens detected in children were norovirus (43%) and rotavirus (18%). Vibrio parahaemolyticus (18%) and rotavirus (17%) were the most common pathogens found in adults. Multiple and mixed infections were common. All noroviruses were identified as genogroup II/type 4(GII/4). The genotype distributions of rotaviruses were G2P[4] (48%), G12P[6] (37%), G2P[6] (5%), G9P[8] (5%), and non-typeable G2 (5%). Conclusions: This study provides preliminary data on the importance of norovirus and rotavirus as diarrhea etiologic agents in Maldives. A systematic prospective diarrhea surveillance documenting disease burden, etiology, seasonal variation, as well as risk factors should be conducted for the development of public health interventions to reduce diarrhea morbidity and mortality in Maldives.