TITLE:
Transforming growth factor-β3 induced rat bone marrow-derived mesenchymal stem cells differentiation into smooth muscle cells by activating Myocardin
AUTHORS:
Lin-Lin Ma, Nan Wang, Zhen Zhou, Jun-Yun Zhang, Xue-Gang Luo, Yong Jiang, Tong-Cun Zhang
KEYWORDS:
Mesenchymal Stem Cells; Smooth Muscle Cells; TGF-β; MRTFs
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.2 No.8,
December
15,
2009
ABSTRACT: Bone marrow mesenchymal stem cells (MSCs) can differentiate into smooth muscle cells (SMCs) and have tremendous potential for cell therapy and tissue engineering. In this study, to understand the effects of TGF-β3 on rat bone marrow-derived MSCs and the underlying molecular mechanism of this differentiation process, we investigated that the changes of myocardin-related transcription factors (MRTFs) at the transcriptional level after rat MSCs were treated with TGF-β3. The results showed that TGF-β3 increased the expression of contractile genes, such as SM22, smooth muscle-myosin heavy chain (SM- MHC), SM-α-actin in MSCs. When TGF-β3 induced MSCs differentiation into SMCs, myocardin and MRTF-A were activated. The data indicated that TGF-β3 induced rat bone marrow-derived MSCs differentiation into SMCs by activating mypcardin and MRTF-A.