TITLE:
Isoflavone Ameliorates H2O2 Induced Injury by Activating the Antioxidant System of Sow Mammary Gland Cell
AUTHORS:
Xianyong Ma, Zongyong Jiang, Jian Zhang, Youjun Hu, Kaiguo Gao, Li Wang, Xuefen Yang
KEYWORDS:
Isoflavone, Antioxidant System, Sow Mammary Gland Cell
JOURNAL NAME:
Natural Science,
Vol.7 No.12,
November
25,
2015
ABSTRACT: The antioxidant and protective properties of a
synthetic soybean isoflavone (ISO) were investigated using sow mammary gland
cell. Cells were cultured with 10, 20 and 30 uM ISO, respectively, under 80 uM
FeSO4·7H2O/H2O2 conditions. After 48 h of incubation, the cells in the presence of ISO were
lost less compared with that of control under oxidative damage by H2O2/FeSO4;
ISO decreased the cell number at G1 and G2 stages, increased the cell number at
S stage (all P P P P P rd to 6th days. Upon these, the activities of total superoxide
dismutase (SOD), glutathione peroxidase (GPX), total antioxidant ability (T-AOC)
also were increased and the activities of NADPH oxidase (NOX) decreased by ISO
treatment (all P P P P P P P P P P P