TITLE:
Normobaric hypoxia-induced brain damage in wistar rat
AUTHORS:
Ding-Yu Hu, Qin Li, Bo Li, Rong-Ji Dai, Li-Na Geng, Yu-Lin Deng
KEYWORDS:
Hypoxia; Brain Damage; HIF-1α; Rat
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.2 No.8,
December
15,
2009
ABSTRACT: The biochemical indicators of wistar rat under low oxygen concentration, such as brain water content, necrosis, lactic acid and Na+-K+-ATPase, was detected to evaluate normobaric hypoxia-induced brain damage and to investigate the mechanism of wistar rat brain injury. Histopathological changes in brain tissue induced by hypoxia were investigated via hematoxylin and eosin stain (HE). Hypoxia induced factor-1α (HIF-1α) expression in brain was confirmed using immunohistochemistry. The results showed that the level of lactic acid was positively correlated with the degree of hypoxia, while concentration-dependent decrease in total Na+-K+-ATPase activity was observed. Compared with the control group, hypoxia group had a significant difference on brain water content under severe hypoxic conditions, the rate of brain necrosis increased obviously, followed by the increase of lactic acid level and the decrease of Na+-K+-ATPase activity. Histopathological analysis of brain confirmed that there was neuronal cell death in hippocampal gyrus. HIF-1α expression enhanced the hypoxia adaptation capability of the rat model through regulating the expressions of multiple genes. Lactic acid, Na+-K+-ATPase and HIF- 1α played an important role in brain injury as a possible mechanism.