TITLE:
The Effect of Sodium Nitrite on Induction of Apoptosis in Human Gastric Adenocarcinoma Epithelia (AGS) Cells
AUTHORS:
Yanchao Liu, Qiliang Qin, Agula Bo, Hairong Zhang, Qing Zhang, Wenli Hao, Yueling Hu, Juan Sun
KEYWORDS:
Sodium Nitrite, Human Gastric Adenocarcinoma Epithelia (AGS) Cells, Apoptosis
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.6 No.11,
November
16,
2015
ABSTRACT: To examine the cytocidal effect of sodium nitrite on the cancer cell, we subjected human gastric adenocarcinoma epithelia (AGS) cells to various experimentation following exposure to sodium nitrite, and measured the resulting changes in the levels of cell death, lactate dehydrogenase (LDH) release, and caspase-3, -6, -8, and -9 activities. Our data revealed that, in AGS cells, treatment with ≥6.25 mM sodium nitrite for 8 h resulted in an obvious increase in cell death. LDH release was also markedly increased following sodium nitrite treatment, but at a concentration of ≥6.25 mM for 24 h. This increasing trend showed a positive correlation (r = 0.9564, P