TITLE:
Evaluation of the Anti-Diabetic Potential of the Methanol Extracts of Aloe camperi, Meriandra dianthera and a Polyherb
AUTHORS:
Mussie Sium Demoz, Kareru Patrick Gachoki, Keriko Joseph Mungai, Berhane Girmay Negusse
KEYWORDS:
Antihyperglycemia, Fasting Blood Glucose Level, Aloe camperi, Meriandra dianthera, Polyherb
JOURNAL NAME:
Journal of Diabetes Mellitus,
Vol.5 No.4,
October
30,
2015
ABSTRACT: The
objective of the study was to evaluate the anti-diabetic activities of methanol
extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a
polyherbal drug (PH) in diabetes induced Wistar albino rats. A single dose of
alloxan monohydrate (150 mg/kg, i.p.) was used to induce diabetes mellitus
(DM). Diabetes was confirmed by the elevated blood glucose levels determined
after 72 h of induction. Animals with mean fasting blood glucose (FBG) level
more than 200 mg/dl were recruited for the experiment. The herbal extracts at
doses of 200 and 400 mg/kg and standard drugmetformin (5 mg/kg) were
administered orally to the diabetic rats for 21 days and the FBG level was
estimated on 0, 7, 14 and 21 days. The herbal extracts showed dose-dependent
fall in FBG levels and the result exhibited very significant (P he highest antihyperglycemic effect was observed by MD extract at 400 mg/kg and was
comparable to the standard drug. Oral glucose tolerance test (OGTT) was
also conducted on normal rats and thus glucose at 2 g/kg per body weight was loaded via oral gavage to all groups 30
min after extract administration. All the groups showed significant increase (P P The hyperglycemia with glucose challenge was
significantly brought down (P herbal extracts at 60 and 120 min relative to the negative
control. Moreover, acute oral toxicity tests was conducted based on the protocols of OECD-425 and
thus the LD50 of the herbal extracts was estimated to be greater than 2000 mg/kg. Statistical analysis was
performed using One-Way ANOVA followed by
Dunnett’s test for multiple comparisons, and values of P