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Tudisco, R., Lombardi, P., Bovera, F., d’Angelo, D., Cutrignelli, M.I., Mastellone, V., Terzi, V., Avallone, L. and Infascelli, F. (2006) Genetically Modified Soya Bean in Rabbit Feeding: Detection of DNA Fragments and Evaluation of Metabolic Effects by Enzymatic Analysis. Animal Science, 82, 193-199.
http://dx.doi.org/10.1079/ASC200530

has been cited by the following article:

• TITLE: Safety Evaluation of Stacked Genetically Modified Corn Event (MON89034 × MON88017) Using Zebrafish as an Animal Model

  AUTHORS: Ahmed M. Rayan, Fikru Nigussie, Louise C. Abbott

  KEYWORDS: Embryonic Development, GM Corn, Safety Evaluation, Transgenic DNA, Zebrafish

  JOURNAL NAME: Food and Nutrition Sciences, Vol.6 No.14, October 29, 2015

  ABSTRACT: This study assessed the effect of feeding stacked genetically modified (GM) corn event (MON89034 × MON88017) on embryonic development, adult tissue histology and assessed the possibility of transgenic DNA transfer, using zebrafish as an animal model. Adult zebrafish were separated into male and female groups. After 3 weeks of feeding with experimental diets, males and females were paired once a week and fertilized embryos were collected, then the male and female fish were re-turned to their sex-specific groups. This fertilization and embryo collection process were repeated 3 times. Embryonic morphological measurements were obtained on developing embryos up to 96 hours after fertilization, and then the embryos were fixed in 4% phosphate-buffered paraformal-dehyde for morphologic assessment. At the end of the feeding study, various adult zebrafish tissues were histologically examined for abnormalities. Both zebrafish tissues and embryos were analyzed for presence of plant specific genes and transgenic sequences. No changes or abnormalities were observed in embryonic morphology nor in any of the tissues examined histologically. In addition, no plant DNA reference genes or transgenic DNA were found in any of the analyzed samples. These results demonstrate that the safety and nutrition of MON89034 × MON88017 corn are similar to non-GM corn.