TITLE:
Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured in Vitro
AUTHORS:
Ahmed Majeed Al-Shammari, Worod Jawad Kadhim Allak, Mahfoodha Umran, Nahi Y. Yaseen, Ayman Hussien
KEYWORDS:
Angiogenesis Factors, Breast Cancer Cell, AMJ13, Tumor Tissue
JOURNAL NAME:
Advances in Breast Cancer Research,
Vol.4 No.4,
September
30,
2015
ABSTRACT: Background: AMJ13 is a new breast cancer cell line
that has been established from a 70-year-old Iraqi woman with a histological
diagnosis of infiltrating ductal carcinoma. It is the first for an Iraqi
population. In breast cancer, angiogenesis provides the tumor tissue,
which is rapidly proliferated with oxygen and nutrients, removes wastes and
increases the opportunity of cancer cells to invade other organs. Methods: The
AMJ13 breast cancer cell line was represented at three different passages and
incubated for interval times. Microarray panel of 43 different angiogenesis
markers was used to scan the supernatant for the factors. ELISA was used to quantify some of the important angiogenesis
factors released in the culture medium and to confirm absence of those who was not
detected by the antibody array. RT-PCR was used
to confirm the gene expression (mRNA) of studied factors. Results: Microarray analysis
showed that TIMP1 and two secreted at highest levels compared to the rest of the factors with low presence of endostatin. Other
non-detectable factors by microarray
examined by ELISA assay that showed highest expression level of VEGF-A were
obtained at earliest passage, while the highest levels of FGF-b were
obtained at late passage. The VEGF-D secretion was shown low concentrations at
all studied passages. There is no detectable level of EGF protein in different
passages and times interval tested. There are no significant differences in
secretion of sICAM between different passages and incubation periods.
Conclusion is that AMJ13 cell line depends on VEGF-A as main angiogenesis
factor to induce micro-vessels supported by low levels of VEGF-D for lymphatic
vessels formation. AMJ13 cell line depends on FGF as growth factors as in late
passages it was shifted to depend mainly on FGF completely. All of this process
may be regulated by TGF-β. TIMP-1 has
proangiogentic effect and has feedback talk with TIMP-2. Understanding the
angiogenesis process for breast cancer can give us better targets for therapy
and more effective treatments.