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Plikaytis, B., Yakrus, M., Butler, W., Woodley, C., Silcox, V. and Shinnick, T. (1992) Differentiation of Slowly Growing Mycobacterium Species, Including Mycobacterium tuberculosis, by Gene Amplification and Restriction Fragment Length Polymorphism Analysis. Journal of Clinical Microbiology, 30, 1815-1822.

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• TITLE: PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba

  AUTHORS: Yaxsier de Armas, Carlos Manuel Fernández, Raúl Díaz, Nicolas Bioni Stefano, Gustavo Wissmann, Enrique José Calderón

  KEYWORDS: Nocardia, PCR-RFLP, Cuba, Molecular Identification, 65-kDa HSP Gene

  JOURNAL NAME: Advances in Microbiology, Vol.5 No.10, September 30, 2015

  ABSTRACT: Nocardiosis diagnosis is a major challenge. The clinical features and radiological findings are nonspecific. Traditionally, Nocardia identification is based on colonial and microscopical morphology and biochemical tests. However, molecular biology techniques allow a better characterization of species and biotypes. PCR-RFLP of the 65-kDa heat shock protein (HSP) gene provides a rapid, sensitive, and time and labor-efficient method for this proposal. Using this technique, six of eight isolates tested were identified as Nocardia asteroides type VI. PCR-RFLP of the 65-kDa HSP gene could be very useful for determining the incidence of this pathogen in different population groups and its association with susceptibility/resistance profiles to the drugs of choice for treatment. This work is the first molecular detection of Nocardia species in Cuba.