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Steingrube, V.A., Brown, B.A., Gibson, J.L., Wilson, R.W., Brown, J., Blacklock, Z., Jost, K., Locke, S., Ulrich, R.F. and Wallace Jr., R.J. (1995) DNA Amplification and Restriction Endonuclease Analysis for Differentiation of 12 Species and Taxa of Nocardia, including Recognition of Four New Taxa within the Nocardia asteroides Complex. Journal of Clinical Microbiology, 33, 3096-3101.

has been cited by the following article:

• TITLE: PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba

  AUTHORS: Yaxsier de Armas, Carlos Manuel Fernández, Raúl Díaz, Nicolas Bioni Stefano, Gustavo Wissmann, Enrique José Calderón

  KEYWORDS: Nocardia, PCR-RFLP, Cuba, Molecular Identification, 65-kDa HSP Gene

  JOURNAL NAME: Advances in Microbiology, Vol.5 No.10, September 30, 2015

  ABSTRACT: Nocardiosis diagnosis is a major challenge. The clinical features and radiological findings are nonspecific. Traditionally, Nocardia identification is based on colonial and microscopical morphology and biochemical tests. However, molecular biology techniques allow a better characterization of species and biotypes. PCR-RFLP of the 65-kDa heat shock protein (HSP) gene provides a rapid, sensitive, and time and labor-efficient method for this proposal. Using this technique, six of eight isolates tested were identified as Nocardia asteroides type VI. PCR-RFLP of the 65-kDa HSP gene could be very useful for determining the incidence of this pathogen in different population groups and its association with susceptibility/resistance profiles to the drugs of choice for treatment. This work is the first molecular detection of Nocardia species in Cuba.