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Condas, L.A.Z., Garcia, M., Domingues, M., de Vargas, A.P.C., Matsuzawa, T., Yazawa, K., Siqueira, A.K., Salerno, T., Lara, G.H.B., Risseti, R.M., Ferreira, K.S. and Gonoi, T. (2014) Molecular Identification and Antimicrobial Resistance Pattern of Seven Clinical Isolates of Nocardia spp. in Brazil. Revista do Instituto de Medicina Tropical de Sao Paulo, 56, 397-401.

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• TITLE: PCR-RFLP Methodology to Identify Nocardia Isolates in Cuba

  AUTHORS: Yaxsier de Armas, Carlos Manuel Fernández, Raúl Díaz, Nicolas Bioni Stefano, Gustavo Wissmann, Enrique José Calderón

  KEYWORDS: Nocardia, PCR-RFLP, Cuba, Molecular Identification, 65-kDa HSP Gene

  JOURNAL NAME: Advances in Microbiology, Vol.5 No.10, September 30, 2015

  ABSTRACT: Nocardiosis diagnosis is a major challenge. The clinical features and radiological findings are nonspecific. Traditionally, Nocardia identification is based on colonial and microscopical morphology and biochemical tests. However, molecular biology techniques allow a better characterization of species and biotypes. PCR-RFLP of the 65-kDa heat shock protein (HSP) gene provides a rapid, sensitive, and time and labor-efficient method for this proposal. Using this technique, six of eight isolates tested were identified as Nocardia asteroides type VI. PCR-RFLP of the 65-kDa HSP gene could be very useful for determining the incidence of this pathogen in different population groups and its association with susceptibility/resistance profiles to the drugs of choice for treatment. This work is the first molecular detection of Nocardia species in Cuba.