TITLE:
Inhibition of Chemically-Induced Colon Cancer by Dietary Treatment of Hibiscus sabdariffa L. Dried Calyx in Rats
AUTHORS:
Rajitha Sunkara, Louis Shackelford, Lloyd Walker, Martha Verghese
KEYWORDS:
Sorrel, Azoxymethane, Colon Cancer, Antioxidant
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.6 No.12,
September
28,
2015
ABSTRACT: This study examined the chemopreventive effect of feeding sorrel calyx as meal and juice against azoxymethane (AOM) induced colon cancer in Fisher 344 male rats. Rats were randomly assigned to five different groups and administered either sorrel meal (5% & 10%) or juice (2.5% & 5%) and control diet. Tumors were induced in rats with two subcutaneous injections (16 mg/kg body weight) of AOM at 6 & 7 weeks of age. Rats were killed at 45 weeks of age and samples (colon, liver) were collected. Tumor incidence, size and numbers were analyzed macroscopically. Activity of drug metabolizing (Cytochrome P2E1 (CYP2E1) & Glutathione S-Transferase (GST)) and antioxidative enzymes (Catalase and Superoxide dismutase) were determined in liver. Dietary feeding of sorrel calyx decreased (P