TITLE:
Effects of Melatonin on Ischemia-Reperfusion Injury in Rat Ovary: Histopathologic and Immunohistochemical Study
AUTHORS:
Deniz Balsak, Cihan Toğrul, Cenap Ekinci, Kerem Doğa Seçkin, Mehmet Fatih Karsli, Aysun Ekinci, Ali Emre Tahaoğlu, Hanefi Bademkiran, Zuat Acar, Senem Yaman Tunç, Engin Deveci, Günsen Kirman
KEYWORDS:
Ischemia-Reperfusion, Ovary, Rat, CD31, CD68
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.5 No.11,
September
24,
2015
ABSTRACT: Aim: To investigate the effect of melatonin on ischemia–reperfusion (I/R)
injury in rat ovary. Material and Method: A total of 24 Wistar rats were
divided into four groups. The rats were first numbered randomly and then
randomly divided into four equal groups: sham, torsion, detorsion and melatonin
groups. In group I (n = 6) sham, as well as in group II ovary torsion was not
performed and no drug was administered. In group III, 1 hour of ischemia and 2
hours of reperfusion were performed and no drug was given. In group IV, melatonin
was dissolved in 1% ethanol just before use. Melatonin was injected 1 hour before
torsion to the torsion plus melatonin group. Right ovaries were surgically
removed in all groups. Result: Malondialdehyde (MDA) levels were measured. We
suggested that administration of melatonin would be in treating ovaries from torsion/detorsion
induced damage in humans. The histopathological changes such as vascular congestion,
edema, hemorrhage, and follicular degeneration were found to be decreased in
the melatonin + ischemia-reperfusion group. Ischemia-reperfusion group: PECAM-I
expression is positive in vessel of the stromal area. The follicles and
interfolicular area infiltrated with CD68 positive cells were increased in a
time of ischemia-reperfusion exposure dependent manner. Conclusions: Melatonin
after ischemia-reperfusion CD31 and CD68 expressions is weak, due to the
reduction of the inflammatory effects and hemorrhage is thought that melatonin
is effective.