TITLE:
Effect of Dissolved Oxygen and Inoculum Concentration on Xylose Reductase Production from Candida guilliermondii Using Sugarcane Bagasse Hemicellulosic Hydrolysate
AUTHORS:
Thais Suzane dos Santos Milessi, Anuj Kumar Chandel, Ricardo de Freitas Branco, Sílvio Silvério da Silva
KEYWORDS:
Xylose Reductase, Sugarcane Bagasse, Candida Guilliermondii, Experimental Design
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.2 No.3,
May
17,
2011
ABSTRACT: This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the parameters were studied under an experimental design 22 with triplicate at central point. The statistical analysis of the results indicated a significant negative effect on XR production from the variable inoculum. The variable dissolved oxygen also showed a negative effect on XR production. We found the maximum enzyme activity (2.5 U?mg?1) when both the factors were applied at their lowest levels. The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source. Also, the results presented important information for further optimization of xylose reductase attainment.