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H. Wu, Z. Song, M. Hentzer, J. B. Anderson, A. Heydorn, K. Mathee, C. Moser, L. Ebrel, S. Molin, N. Hoiby and M. Givskov, “Detection of N-acylhomoserine Lactones in Lung Tissues of Mice Infected with Pseudomonas Aeruginosa,” Microbiology, Vol. 146, No. 10, 2000, pp. 2481- 2493.

has been cited by the following article:

• TITLE: Occurrence of N-Acyl Homoserine Lactones in Extracts of Bacterial Strain of Pseudomonas aeruginosa and in Sputum Sample Evaluated by Gas Chromatography–Mass Spectrometry

  AUTHORS: Susheela Rani, Ashwini Kumar, Ashok Kumar Malik, P. Schmitt-Kopplin

  KEYWORDS: Gas Chromatography—Mass Spectrometry, N-Acyl Homoserine Lactone (N-Butanoyl, N-Hexanoyl, N-Octanoyl, N-Decanoyl, N-Dodecanoyl and N-Tetradecanoyl) Homoserine Lactone, Sputum Sample, Bacterial Strain

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.2 No.2, May 30, 2011

  ABSTRACT: This study presents a fast, accurate and sensitive technique using gas chromatography-mass spectrometry (GC-MS) for the identification and quantification of N-acyl homoserine lactones (AHLs) in the extracts of bacterial strain of Pseudomonas aeruginosa and sputum sample of a cystic fibrosis patient. This method involves direct separation and determination of AHLs by using GC-MS as simultaneous separation and characterization of AHLs were possible without any prior derivatiza-tion. Electron ionization resulted in a common fragmentation pattern with the most common fragment ion at m/z 143 and other minor peaks at 73, 57 and 43. The limit of detection for N-butanoyl, N-hexanoyl, N-octanoyl, N-decanoyl, N-dodecanoyl and N-tetradecanoyl homoserine lactones was 2.14, 3.59, 2.71, 2.10, 2.45 and 2.34 µg/L, respectively. The presence of AHLs in the culture of P. aeruginosa strain and spu-tum of a cystic fibrosis patient was achieved in selected ion monitoring (SIM) mode by using the prominent fragment at m/z 143.