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P. Kintz and P. Mangin, “What Constitutes a Positive Result in Hair Analysis: Proposal for the Establishment of Cut-off Values,” Forensic Science International, Vol. 70, No. 1, January 1995, pp. 3-11. doi:10.1016/0379-0738(94)01621-B

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• TITLE: Liquid Chromatographic Determination of Scopolamine in Hair with Suspended Drop Liquid Phase Microextraction Technique

  AUTHORS: Mahboubeh Masrournia, Zarrin Es’ haghi, Mostafa Amini

  KEYWORDS: Column Liquid Chromatography, Directly Suspended Droplet Liquid Phase Microextraction (SDLME), Scopolamine, Hair Sample

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.2 No.2, May 30, 2011

  ABSTRACT: Hair analysis is used in some branches of alternative medicine as a method of investigation to assist diagnosis. It is very useful when a history of drug use is difficult or impossible to obtain. In this re-search suspended droplet liquid phase microextraction (SDLME) coupled with high-performance liquid chromatography and photodiode array detection (HPLC-DAD) was used for preconcentration and analysis of scopolamine in hair samples. Therefore scopolamine was extracted from 2.0 g hair sample incubated in methanol (5 h, 50°C) and adjusted to pH 7.4 with, Na2HPO4–H3PO4 buffer solution (donor phase, P1) into an organic phase (P2) 350 µl n-octanol and then back extracted into a micro drop of aqueous acceptor phase (P3), adjusted at pH 3, with HCL. The extraction time, T1 (from P1 to P2) was 2 min and T2 (from P2 to P3) was 30 min. Optimum instrumental conditions were included; A C18 reverse phase column with water-acetonitrile-methanol (80:10:10) as the mobile phase was used and wavelength for UV detec-tion was 205 nm. The linear range was 10 to 10000 ng●mL–1, enrichment factor, detec-tion limit and relative standard deviation were 77, 0.1 ng●mL–1 and 5.4 respectively.