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ISO 21570, Organizaçao Internacional De Normalizaçao (2005) Foodstuffs: Methods of Analysis for the Detection of Genetically Modified Organisms and Derived Products—Quantitative Nucleic Acid Based Methods. ISO, Switzerland, 103 p.

has been cited by the following article:

• TITLE: Isoflavone and Mineral Content in Conventional and Transgenic Soybean Cultivars

  AUTHORS: Gabriela Rezende Costa, Nilton de Oliveira Couto e Silva, José Marcos Gontijo Mandarino, Rodrigo Santos Leite, Nilson César Castanheira Guimarães, Roberto Gonçalves Junqueira, Renata Adriana Labanca

  KEYWORDS: Glycine max, Genetically Modified Organism, Bioactive Compounds, ICP-OES, HPLC

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.13, August 26, 2015

  ABSTRACT: The objective of this study was to evaluate the differences in composition among six brands of conventional soybean and six genetically modified cultivars (GM). We focused on the isoflavones profile and mineral content questioning the substantial equivalence between conventional and GM organisms. The statement of compliance label for conventional grains was verified for the presence of genetic modified genes by real time polymerase chain reaction (PCR). We did not detect the presence of the 35S promoter in commercial samples, indicating the absence of transgene insertion. For mineral analysis, we used the method of inductively coupled plasma-optical emission spectrometry (ICP-OES). Isoflavones quantification was performed by high performance liquid chromatography (HPLC). The results showed no statistical difference between the conventional and transgenic soybean groups concerning isoflavone content and mineral composition. The concentration of potassium, the main mineral component of soy, was the highest in conventional soybeans compared to that in GM soy, while GM samples presented the highest concentrations of iron.